chip dna clean & concentrator (Zymo Research)
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Chip Dna Clean & Concentrator, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 1600 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/chip+dna+clean+and+concentrator/ChIP+DNA+Clean+%26+Concentrator/custom%40d5201%4042791404
Average 99 stars, based on 1600 article reviews
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Isolation:Article Title: Stage-specific epigenetic priming amplifies gene activation during lineage commitment. Article Snippet: .. DNA was isolated using Zymo Article Title: Stage-specific epigenetic priming amplifies gene activation during lineage commitment Article Snippet: .. DNA was isolated using Zymo Chromatin Immunoprecipitation:Article Title: Stage-specific epigenetic priming amplifies gene activation during lineage commitment. Article Snippet: .. DNA was isolated using Zymo Article Title: H3.1K27M-induced misregulation of the TONSOKU-H3.1 pathway causes genomic instability Article Snippet: .. After uncrosslinking, samples were treated with proteinase K and purified using Article Title: H3.1K27M-induced misregulation of the TONSOKU-H3.1 pathway causes genomic instability. Article Snippet: .. After uncrosslinking, samples were treated with proteinase K and purified using Article Title: The DLX/Notch axis is necessary for spatiotemporal regulation of neural cell fate Article Snippet: Eluted fragments were incubated with 0.01M EDTA, 0.04M Tri-HCl, pH 7.4, 0.1 mg/mL Proteinase K (Promega Cat# MC5008) at 50°C for 2 hours for crosslinking reversal. .. The eluted DNA fragments were purified using the Article Title: Stage-specific epigenetic priming amplifies gene activation during lineage commitment Article Snippet: Transposition was carried out in 50 μl of transposition mix [25 μl of 2× TD buffer (Illumina), 2.5 μl of transposase (Illumina), 16.5 μl of 1× PBS, 0.5 μl of 1% digitonin, 0.5 μl of 10% Tween 20, and 5 μl of water) at 37°C for 30 min in a thermomixer with shaking at 1000 rpm. .. Transposed DNA was collected using the Zymo Article Title: Stage-specific epigenetic priming amplifies gene activation during lineage commitment Article Snippet: .. DNA was isolated using Zymo Purification:Article Title: H3.1K27M-induced misregulation of the TONSOKU-H3.1 pathway causes genomic instability Article Snippet: .. After uncrosslinking, samples were treated with proteinase K and purified using Article Title: H3.1K27M-induced misregulation of the TONSOKU-H3.1 pathway causes genomic instability. Article Snippet: .. After uncrosslinking, samples were treated with proteinase K and purified using Article Title: The DLX/Notch axis is necessary for spatiotemporal regulation of neural cell fate Article Snippet: Eluted fragments were incubated with 0.01M EDTA, 0.04M Tri-HCl, pH 7.4, 0.1 mg/mL Proteinase K (Promega Cat# MC5008) at 50°C for 2 hours for crosslinking reversal. .. The eluted DNA fragments were purified using the Incubation:Article Title: Conserved HSFA1-dependent chromatin dynamics drive heat stress responses in plants. Article Snippet: The nuclei were then pelleted by centrifugation at 1000 × g for 10 min at 4 ◦ C. The sorted nuclei were resuspended in a 50 μL tagmentation reaction mixture consisting of 25 μL of 2x TD buffer, 2.5 μL of TDE1 enzyme (Illumina Tagment DNA TDE1 Enzyme and Buffer Kits), and 22.5 μL of nuclease-free water. .. The reaction was incubated at 37 ◦ C for 30 min, followed by DNA purification with Zymo DNA Purification:Article Title: Conserved HSFA1-dependent chromatin dynamics drive heat stress responses in plants. Article Snippet: The nuclei were then pelleted by centrifugation at 1000 × g for 10 min at 4 ◦ C. The sorted nuclei were resuspended in a 50 μL tagmentation reaction mixture consisting of 25 μL of 2x TD buffer, 2.5 μL of TDE1 enzyme (Illumina Tagment DNA TDE1 Enzyme and Buffer Kits), and 22.5 μL of nuclease-free water. .. The reaction was incubated at 37 ◦ C for 30 min, followed by DNA purification with Zymo other:Article Title: Stage-specific epigenetic priming amplifies gene activation during lineage commitment. Article Snippet: Two million to 4 million cells were resuspended in 1 ml of lysis buffer [10 mM tris- HCl (pH 8), 10 mM NaCl, and 0.2% Igepal CA- 630], and nuclei were extracted via sonication using a Covaris E220 sonicator (75- W peak incident power, 2% duty factor, 200 cycles per burst, and 45 s). Polymerase Chain Reaction:Article Title: Stage-specific epigenetic priming amplifies gene activation during lineage commitment Article Snippet: Transposition was carried out in 50 μl of transposition mix [25 μl of 2× TD buffer (Illumina), 2.5 μl of transposase (Illumina), 16.5 μl of 1× PBS, 0.5 μl of 1% digitonin, 0.5 μl of 10% Tween 20, and 5 μl of water) at 37°C for 30 min in a thermomixer with shaking at 1000 rpm. .. Transposed DNA was collected using the Zymo |
